Q: The cells on this IFA slide all appear positive. Is this possible?
A: If all the cells are positive
this is likely to be some type of an anti-cellular reaction. The percent positive should
be approximately the same as seen with the positive control.
Q: When
only two or three cells are seen fluorescing is this a positive?
A: No, the percent of positive
cells seen should be at least as high as the number of positive cells seen with the
positive control at its endpoint.
Q: Why is
the positive control for this IFA kit barely positive at the screening dilution?
A: The positive controls are
bottled at the screening dilution and can be titered for an endpoint from this dilution.
The control should be used in the first well without further dilution.
Q: Is it
possible to differentiate the restricted and diffuse antigen using these slides?
A: The EA slides do not
differentiate between the two types of antigen.
Q: Why
does this kit have a sample diluent?
A: During the development of this
kit it was found that a diluent improved the readability of the slides and decreased the
non-specific reactivity. |
![[Picture of EBV Early slide]](../graphics/slide15.jpg)
![[Picture of EBV Early slide]](../graphics/slide16.jpg)
